7DCB
The structure of the Arabidopsis thaliana guanosine deaminase complexed with inosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2020-04-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 117.980, 117.980, 37.736 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.060 - 2.000 |
R-factor | 0.1663 |
Rwork | 0.165 |
R-free | 0.19750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dbf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.681 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.223 | 0.759 |
Number of reflections | 20634 | 1963 |
<I/σ(I)> | 19.2 | 2.5 |
Completeness [%] | 99.5 | 97.5 |
Redundancy | 18.6 | 13.8 |
CC(1/2) | 0.967 | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |