7DCA
The structure of the Arabidopsis thaliana guanosine deaminase bound by xanthosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-05 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 119.350, 119.350, 39.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.670 - 2.100 |
R-factor | 0.1743 |
Rwork | 0.172 |
R-free | 0.21990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dbf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.711 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.672 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.170 | 0.806 |
Number of reflections | 19223 | 2785 |
<I/σ(I)> | 12.1 | 2.4 |
Completeness [%] | 99.9 | |
Redundancy | 8.6 | 5.6 |
CC(1/2) | 0.992 | 0.689 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |