7DBO

DPBB domain of VCP-like ATPase from Thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-05
Detector	RAYONIX MX225-HS
Wavelength(s)	0.979000
Spacegroup name	H 3 2
Unit cell lengths	118.800, 118.800, 68.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.070 - 1.900
R-factor	0.2217
Rwork	0.217
R-free	0.26420
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	14642	2292
<I/σ(I)>	7.69	1.21
Completeness [%]	99.7
Redundancy	22.1
CC(1/2)	0.995	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM citrate / phosphate pH5.0, 1.4M Ammonium sulfate