7DBN

HIV-1 reverse transcriptase mutant Q151M/Y115F/F116Y/M184V/F160M:DNA:dCTP ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	H 3
Unit cell lengths	285.681, 285.681, 96.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.895 - 2.670
R-factor	0.1791
Rwork	0.177
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kdj
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.670	2.670
Rmeas	0.089	0.982
Number of reflections	83121	4525
<I/σ(I)>	14.8	2
Completeness [%]	100.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	BIS-TRIS-HCL, DI-AMMONIUM HYDROGEN CITRATE, MGCL2, PEG 6000, SUCROSE, GLYCEROL