7DBF
The structure of the Arabidopsis thaliana guanosine deaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 119.117, 119.117, 39.453 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.610 - 1.900 |
R-factor | 0.1677 |
Rwork | 0.166 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a8n |
RMSD bond length | 0.006 |
RMSD bond angle | 0.733 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.897 | 1.900 |
Rmerge | 0.128 | 0.815 |
Number of reflections | 25636 | 1273 |
<I/σ(I)> | 30.9 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.4 | 9.7 |
CC(1/2) | 0.989 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |