7DAA

Crystal structure of basigin complexed with anti-basigin Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	117.345, 247.352, 52.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.676 - 2.510
R-factor	0.2305
Rwork	0.228
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d9z
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	pointless (1.12.2)
Data scaling software	autoPROC
Phasing software	MOLREP (11.7.03)
Refinement software	PHENIX (1.19rc2_4022)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.676	2.706
High resolution limit [Å]	2.509	2.509
Number of reflections	18245	571
<I/σ(I)>	7.3
Completeness [%]	86.3
Redundancy	5.1
CC(1/2)	0.993	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Tris-HCl (pH 8.0) buffer containing 0.1M sodium chloride, 0.1M cadmium chloride hemi(pentahydrate), 33% PEG 400