7DA1

Crystal structure of the chicken MHF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	59.432, 59.432, 220.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.460 - 2.010
R-factor	0.1987
Rwork	0.197
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b0b
RMSD bond length	0.007
RMSD bond angle	0.909
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.460	2.082
High resolution limit [Å]	2.010	2.010
Rmerge	0.103	0.772
Rmeas	0.108	0.802
Rpim	0.030	0.217
Number of reflections	27614	2661
<I/σ(I)>	22.3	3
Completeness [%]	99.8
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M MOPS-HEPES pH 7.0, 0.1 M Carboxylic acids mix, 15% MPD, 20% PEG3350