7D9J
SpdH Spermidine dehydrogenase Y443A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97737 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.051, 85.501, 101.109 |
Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
Resolution | 34.960 - 2.190 |
R-factor | 0.1494 |
Rwork | 0.147 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d9f |
RMSD bond length | 0.008 |
RMSD bond angle | 0.906 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.190 | 2.190 |
Rpim | 0.040 | 0.144 |
Number of reflections | 59220 | 2898 |
<I/σ(I)> | 18.2 | 5 |
Completeness [%] | 99.2 | 97.7 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1M Bis Tris pH6.5, 20% w/v polyethylene glycol (PEG) 3350 |