7D97
Crystal structure of N109P mutant of GATase subunit of Methanocaldococcus jannaschii GMP synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-02-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.500, 68.050, 75.350 |
| Unit cell angles | 90.95, 90.47, 91.45 |
Refinement procedure
| Resolution | 35.490 - 1.890 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d40 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.624 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 2.030 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 48293 | 5717 |
| <I/σ(I)> | 1.28 | |
| Completeness [%] | 93.7 | |
| Redundancy | 2.1 | |
| CC(1/2) | 0.861 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 277 | 100 mM Sodium acetate, pH 5.6, 20 % PEG 3350 |
