7D8V

Crystal Structure of A Kinesin-3 KIF13B mutant-T192Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	87.510, 87.510, 97.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.920 - 2.300
R-factor	0.1848
Rwork	0.182
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a1z
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.560	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.143	1.059
Number of reflections	19638	961
<I/σ(I)>	10.4	2.1
Completeness [%]	100.0	100
Redundancy	10.8	9.7
CC(1/2)	0.997	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	289	0.1M Bis-Tris propane, 30% (w/v) PEG 20000