7D8R

MITF HLHLZ structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 1
Unit cell lengths	41.910, 78.120, 80.590
Unit cell angles	93.18, 105.03, 105.62

Refinement procedure

Resolution	38.693 - 3.000
R-factor	0.2866
Rwork	0.285
R-free	0.31820
Structure solution method	SAD
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.124	38.693	3.160
High resolution limit [Å]	3.000	9.490	3.000
Rmerge		0.037	0.586
Rmeas	0.061	0.043	0.678
Rpim	0.031	0.023	0.341
Total number of observations	72600	2176	10427
Number of reflections	18512	582	2651
<I/σ(I)>	12.7	28.7	2.4
Completeness [%]	98.3	95.5	98
Redundancy	3.9	3.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	HEPES, KCl, PO/OH