7D89
Crystal structure of an inactivated double mutant (E182AE280A) of a novel thermostable GH10 xylanase XynA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 110.193, 110.193, 84.983 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.280 - 2.894 |
R-factor | 0.234647402838 |
Rwork | 0.232 |
R-free | 0.27908 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v0l |
RMSD bond length | 0.009 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.280 | 2.997 |
High resolution limit [Å] | 2.890 | 2.894 |
Rmerge | 0.108 | 0.108 |
Rmeas | 0.108 | 0.108 |
Rpim | 0.021 | 0.021 |
Number of reflections | 12123 | 12123 |
<I/σ(I)> | 34 | |
Completeness [%] | 99.6 | |
Redundancy | 25.5 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | 24% PEG 600, 0.1M CaCl2, 0.1M MES pH 6.0 |