7D85

Crystal structure of anti-ErbB3 Fab ISU104 in complex with human ErbB3 extracellular domain 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	92.290, 77.780, 93.940
Unit cell angles	90.00, 113.41, 90.00

Refinement procedure

Resolution	46.630 - 2.500
R-factor	0.2043
Rwork	0.203
R-free	0.25840
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.630	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.097	0.537
Number of reflections	42145	4195
<I/σ(I)>	6.56	1.32
Completeness [%]	99.2	99.79
Redundancy	2	2
CC(1/2)	0.987	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M Sodium acetate, 20%(w/v) PEG 3350