7D85
Crystal structure of anti-ErbB3 Fab ISU104 in complex with human ErbB3 extracellular domain 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.290, 77.780, 93.940 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.500 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.25840 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.630 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.537 |
| Number of reflections | 42145 | 4195 |
| <I/σ(I)> | 6.56 | 1.32 |
| Completeness [%] | 99.2 | 99.79 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.987 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium acetate, 20%(w/v) PEG 3350 |
