7D7P

Crystal structure of the phosphodiesterase domain of Salpingoeca rosetta rhodopsin phosphodiesterase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	117.200, 67.540, 56.890
Unit cell angles	90.00, 110.82, 90.00

Refinement procedure

Resolution	47.516 - 2.100
R-factor	0.1907
Rwork	0.188
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vyd
RMSD bond length	0.002
RMSD bond angle	0.418
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	2.180
High resolution limit [Å]	2.100	2.100
Rmeas	0.162	0.798
Number of reflections	22066	1965
<I/σ(I)>	11.4
Completeness [%]	90.7
Redundancy	11.6
CC(1/2)	0.992	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	29% (v/v) PEG 400, 50mM calcium acetate, pH 5.0, 200mM NaCl