7D66
Crystal structure of retroviral protease-like domain of Ddi1 from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 184.601, 184.601, 184.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.266 - 2.126 |
| Rwork | 0.192 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1a |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.833 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.270 | 2.182 |
| High resolution limit [Å] | 2.120 | 2.126 |
| Number of reflections | 176969 | 8844 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.990 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 2 M ammonium citrate tribasic, 30 % 2-propanol |
