7D5Q
Structure of NorC transporter (K398A mutant) in an outward-open conformation in complex with a single-chain Indian camelid antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.071, 139.148, 115.555 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 111.520 - 3.600 |
| R-factor | 0.30802 |
| Rwork | 0.308 |
| R-free | 0.31463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d5p |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.235 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.150 | 3.840 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.177 | 2.760 |
| Rpim | 0.043 | 0.880 |
| Number of reflections | 24925 | 4460 |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 17.1 | 10.1 |
| CC(1/2) | 0.997 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M HEPES pH 6.0, 50 mM NaCl, 57.1 mM CaCl2, 35.7% PEG600, 10 mM YCl3, 6.66 mM CHAPSO |
