7D4B
Crystal structure of 4-1BB in complex with a VHH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 106.851, 106.851, 146.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.790 | 3.360 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.162 | 1.241 |
Rpim | 0.047 | 0.359 |
Number of reflections | 15429 | 2723 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 12.9 | 12.8 |
CC(1/2) | 0.998 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 1.5 M lithium sulfate, 100 mM Tris (pH 8.5), 10 mM nickel sulfate |