7D4A

The Crystal Structure of human JMJD2A Tudor domain from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976253
Spacegroup name	I 41 3 2
Unit cell lengths	135.110, 135.110, 135.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.219 - 2.201
Rwork	0.230
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gf7
RMSD bond length	0.004
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.560	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.129	2.749
Number of reflections	11007	836
<I/σ(I)>	21.4
Completeness [%]	99.9
Redundancy	41.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M NaCl, 0.1M HEPES pH7.5, 1.6M (NH4)2SO4