7D48
apo-form cyclic trinucleotide synthase CdnD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.924, 52.924, 344.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.010 - 2.150 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.593 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.220 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.096 | 0.403 |
| Number of reflections | 27647 | |
| <I/σ(I)> | 17 | 2.7 |
| Completeness [%] | 98.9 | 97.5 |
| Redundancy | 8.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% v/v Tacsimate, pH 6.0, 20% w/v PEG 3350 |
