7D2M
Crystal structure of MazF (Form-II) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2019-10-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.451, 72.451, 112.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.910 - 1.790 |
R-factor | 0.1576 |
Rwork | 0.156 |
R-free | 0.18050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d28 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 23.000 | 23.000 | 1.830 |
High resolution limit [Å] | 1.790 | 4.870 | 1.800 |
Rmerge | 0.181 | 0.097 | 0.407 |
Rmeas | 0.186 | 0.100 | 0.418 |
Rpim | 0.038 | 0.022 | 0.094 |
Number of reflections | 28465 | 1577 | 1356 |
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.4 | 98.3 | 99.1 |
Redundancy | 20 | 20.8 | 17.9 |
CC(1/2) | 0.992 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Lithium Sulfate monohydrate, Tris HCl pH 8.5 and 30% PEG 4,000 |