7D2H
Tetrameric coiled-coil structure of liprin-alpha2_H2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.879, 101.907, 37.445 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 30.051 - 2.200 |
| R-factor | 0.2313 |
| Rwork | 0.229 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d2g |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.080 | 0.042 | 0.847 |
| Rmeas | 0.092 | 0.048 | 0.980 |
| Rpim | 0.044 | 0.023 | 0.483 |
| Total number of observations | 43572 | ||
| Number of reflections | 11895 | 580 | 599 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 93.1 | 87.1 | 94.3 |
| Redundancy | 3.7 | 3.7 | 3.6 |
| CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% v/v 2-Propanol, 0.1 M Tris pH 8.0, 5% w/v Polyethylene glycol 8,000 |
