7D2E

Tetrameric coiled-coil structure of liprin-alpha2_H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	38.307, 71.293, 53.473
Unit cell angles	90.00, 103.69, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.2409
Rwork	0.239
R-free	0.26850
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.758
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.037	0.022	0.757
Rmeas	0.043	0.026	0.886
Rpim	0.022	0.013	0.457
Number of reflections	30192	1259	1528
<I/σ(I)>	15.6
Completeness [%]	98.4	79	99.5
Redundancy	3.7	3.6	3.7
CC(1/2)		0.997	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6,000