7D27
Structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 104.15 |
Detector technology | PIXEL |
Collection date | 2020-05-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 119.960, 119.960, 116.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.001 - 2.480 |
Rwork | 0.228 |
R-free | 0.25980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bub |
RMSD bond length | 0.015 |
RMSD bond angle | 1.776 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.180 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rpim | 0.023 | 0.424 |
Number of reflections | 30793 | 3022 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 100.0 | |
Redundancy | 17.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21%(v/v) 2-propanl, 30% (v/v) Glyceol 0.07M sodium cacodylate trihydrate pH 6.5, 0.14M Sodium citrate tribasic dihydrate |