7D1G

Crystal structure of Glyceraldehyde-3-Phosphate Dehydrogenase GAPDH from Clostridium beijerinckii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 62 2 2
Unit cell lengths	120.640, 120.640, 122.135
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.910 - 1.580
R-factor	0.194
Rwork	0.193
R-free	0.21130
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.580	1.580
Number of reflections	71898	2218
<I/σ(I)>	34.22
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.1 M HEPES sodium pH 8.5 0.2 M Magnesium chloride hexahydrate 30% v/v Polyethylene glycol 400