7D1B
Crystal structure of Fimbriiglobus ruber glutaminyl cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.206, 73.436, 87.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.967 - 1.240 |
| R-factor | 0.1113 |
| Rwork | 0.111 |
| R-free | 0.12540 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.280 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.041 | 0.348 |
| Number of reflections | 102036 | 9674 |
| <I/σ(I)> | 41.8 | 5.1 |
| Completeness [%] | 97.4 | 93.6 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 20% (w/v) PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.1 M MgCl2, 20% (v/v) PEG 400 |
