7D0R

Crystal structure of human HBO1-BRPF2 in complex with crotonoyl-coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	127.345, 39.636, 88.096
Unit cell angles	90.00, 123.24, 90.00

Refinement procedure

Resolution	50.010 - 1.950
R-factor	0.1848
Rwork	0.182
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gk9
RMSD bond length	0.009
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	0.700
Number of reflections	26858	2647
<I/σ(I)>	38.5	2.3
Completeness [%]	97.8
Redundancy	6.2
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.03 M Citric acid, 0.07 M BIS-TRIS propane, pH 7.6, 20% w/v PEG 3350