7CZG

Crystal structure of FIP200 Claw domain apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	64.544, 75.616, 99.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.370 - 1.800
R-factor	0.1912
Rwork	0.189
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dce
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.170	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	41526	5232
<I/σ(I)>	16.7
Completeness [%]	90.6
Redundancy	12.9
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Sodium citrate tribasic dihydrate pH 5.0, Jeffamine ED-2001 pH 7.0