7CWP
Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.200, 66.700, 168.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.994 - 2.100 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.22550 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.890 | 42.890 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.115 | 0.047 | 0.484 |
| Rmeas | 0.129 | 0.054 | 0.541 |
| Rpim | 0.055 | 0.025 | 0.234 |
| Total number of observations | 131390 | 1711 | 10730 |
| Number of reflections | 29074 | 404 | 2413 |
| <I/σ(I)> | 9.4 | 19.6 | 3.1 |
| Completeness [%] | 95.8 | 87.4 | 97.1 |
| Redundancy | 4.5 | 4.2 | 4.4 |
| CC(1/2) | 0.994 | 0.996 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.9 M sodium malonate pH 6.5, 5 mM glucose, 5% glycerol |
