7CWI

Crystal structure of beta-galactosidase II from Bacillus circulans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 32 2 1
Unit cell lengths	159.802, 159.802, 96.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.770 - 1.950
R-factor	0.1574
Rwork	0.156
R-free	0.18690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ypj
RMSD bond length	0.013
RMSD bond angle	1.734
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	5.290	1.980
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.084	0.051	0.299
Rmeas	0.088	0.054	0.326
Rpim	0.028	0.016	0.126
Total number of observations	848695
Number of reflections	101473	5183	4682
<I/σ(I)>	12.3
Completeness [%]	99.2	99.9	92.4
Redundancy	8.4	10.4	5.6
CC(1/2)		0.998	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium sulfate, Sodium acetate/Acetic acid pH4, Sodium Chloride