7CV9

RNA methyltransferase METTL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97776
Spacegroup name	P 1 21 1
Unit cell lengths	63.603, 93.545, 96.612
Unit cell angles	90.00, 104.78, 90.00

Refinement procedure

Resolution	30.000 - 2.455
R-factor	0.2133
Rwork	0.211
R-free	0.25410
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.974
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.030	30.000	2.540
High resolution limit [Å]	2.450	5.270	2.450
Rmerge	0.104	0.074	0.392
Rmeas	0.114	0.080	0.442
Rpim	0.046	0.031	0.198
Number of reflections	39010	4075	3536
<I/σ(I)>	5.7
Completeness [%]	97.6	99.9	88.6
Redundancy	5.3	6.5	4
CC(1/2)		0.991	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1600 mM Magnesium sulfate 100 mM MES/ Sodium hydroxide pH 6.5