7CV1

Structure of human tRNAHis guanylyltransferase (Thg1) in the presence of human mitochondrial tRNAHis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 31 2 1
Unit cell lengths	155.878, 155.878, 201.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.462 - 4.000
R-factor	0.2436
Rwork	0.242
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ote
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.462	49.460	4.280
High resolution limit [Å]	4.000	11.310	4.000
Rmerge	1.011	0.096	7.893
Rmeas	1.036	0.099	8.085
Rpim	0.226	0.022	1.742
Total number of observations		22714	93005
Number of reflections	24468	1173	4363
<I/σ(I)>	5.9	21.5	1.2
Completeness [%]	100.0	98.9	100
Redundancy	20.4	19.4	21.3
CC(1/2)	0.989	0.997	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 7.0, 10% (w/v) PEG6000, 5% (w/v) Tacsimate pH7.0