7CUY

Crystal structure of Primo-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2020-07-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	45.921, 59.902, 60.925
Unit cell angles	90.00, 110.23, 90.00

Refinement procedure

Resolution	29.810 - 2.081
R-factor	0.1855
Rwork	0.179
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5got
RMSD bond length	0.008
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.081	2.081
Number of reflections	18049	839
<I/σ(I)>	23.8
Completeness [%]	97.4
Redundancy	4.7
CC(1/2)	0.983	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES-NaOH (pH 7.47), 1.2 M sodium citrate tribasic, 9% (w/v) D-sorbitol