7CTP
Crystal Structure of Human FAM129B/MINERVA/NIBAN2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-02 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.559, 56.283, 201.007 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.110 - 1.800 |
Rwork | 0.204 |
R-free | 0.23550 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.769 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 59778 | 5794 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.9 | |
Redundancy | 7.5 | |
CC(1/2) | 0.998 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M lithium acetate, 20% (w/v) PEG 3350 |