7CSY

Pseudomonas aeruginosa antitoxin HigA with higBA promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97930
Spacegroup name	C 1 2 1
Unit cell lengths	133.255, 100.433, 68.795
Unit cell angles	90.00, 114.21, 90.00

Refinement procedure

Resolution	29.660 - 2.290
R-factor	0.2113
Rwork	0.209
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f8h
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.660	2.368
High resolution limit [Å]	2.290	2.290
Number of reflections	71719	3415
<I/σ(I)>	33.8
Completeness [%]	98.8
Redundancy	5.6
CC(1/2)	0.596	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	35% (v/v) 2-methyl-2,4-pentanediol, 0.1M HEPES pH7.5, 0.1M NaCl