7CSA
AtPrR1 with NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-03-12 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.265, 144.311, 77.260 |
| Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
| Resolution | 33.295 - 1.962 |
| R-factor | 0.187662060864 |
| Rwork | 0.187 |
| R-free | 0.23509 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.296 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.099 | 0.977 |
| Number of reflections | 100651 | 8293 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M sodium fluoride, 20% w/v PEG 3350 |
