7CRE

hnRNPK KH3 domain in complex with a ssDNA fragment from the SIRLOIN element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 62 2 2
Unit cell lengths	61.611, 61.611, 144.544
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.26
Rwork	0.258
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zzi
RMSD bond length	0.004
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.151	0.163
Rmeas	0.159	0.172
Rpim	0.049	0.054	0.889
Total number of observations	42330
Number of reflections	3670	424	356
<I/σ(I)>	13.7
Completeness [%]	98.8	94.9	100
Redundancy	11.5	9.9	11.1
CC(1/2)		0.979	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25 % w/v Polyethylene glycol 3350, 100 mM BIS-TRIS pH 5.5, 200 mM Ammonium acetate