7CQL
Apo GmaS without ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 115.909, 179.024, 192.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.573 - 2.801 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lni |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.034 | 2.800 |
| Rmerge | 0.128 | 0.073 | 0.310 |
| Rmeas | 0.134 | 0.077 | 0.325 |
| Rpim | 0.040 | 0.022 | 0.099 |
| Total number of observations | 545098 | ||
| Number of reflections | 48331 | 5064 | 4782 |
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 98.6 | 98.8 | 99.1 |
| Redundancy | 11.3 | 12.1 | 10.7 |
| CC(1/2) | 0.998 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES (pH 6.5), 1 M lithium sulfate |
