7CQ4

Crystal structure of Slx1-Slx4 in complex with 5'flap DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 65 2 2
Unit cell lengths	123.223, 123.223, 233.576
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.314 - 3.294
R-factor	0.2369
Rwork	0.235
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cq2
RMSD bond length	0.004
RMSD bond angle	0.993
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.294	7.100	3.300
Rmerge	0.122	0.060	0.722
Rmeas	0.128	0.064	0.758
Rpim	0.039	0.020	0.228
Number of reflections	16540	1811	1587
<I/σ(I)>	7.1
Completeness [%]	99.8	98	100
Redundancy	10.9	9.9	11.1
CC(1/2)		0.997	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	0.1 M sodium cacodylate, pH 6.0, 12% PEG 1500 and 0.1 M TCEP hydrochloride