7CPH
Crystal structure of tRNA adenosine deaminase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.530, 41.250, 202.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.190 - 2.300 |
| R-factor | 0.1811 |
| Rwork | 0.177 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.520 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.126 | 0.514 |
| Rmeas | 0.142 | 0.574 |
| Rpim | 0.064 | 0.248 |
| Number of reflections | 13799 | 2067 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 91.5 | 95.4 |
| Redundancy | 4.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% tacsimate pH 6.0, 20% PEG3350 |
