7CPC

His-Mediated Reversible Self-assembly of Ferritin Nanocage with Ni binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	I 4
Unit cell lengths	125.845, 125.845, 176.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.275 - 2.801
R-factor	0.199
Rwork	0.195
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kh1
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	33438	1668
<I/σ(I)>	7.545
Completeness [%]	99.3
Redundancy	4.6
CC(1/2)	0.959	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	1.26 M (NH4)2SO4, 100 mM Tris (pH =8.0)