7COE

Crystal structure of Receptor binding domain of MERS-CoV and KNIH90-F1 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	71.590, 146.819, 81.622
Unit cell angles	90.00, 96.73, 90.00

Refinement procedure

Resolution	44.950 - 2.050
Rwork	0.176
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zs6
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.950	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	103522	9762
<I/σ(I)>	14.97
Completeness [%]	99.0
Redundancy	7
CC(1/2)	0.956	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	0.1M MES monohydrate pH 6.3, 15%(w/v) PEG 20000