7COC

Ternary complex of DNA polymerase Mu (K438A/Q441A) with 1-nt gapped DNA (T:dGMPNPP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.960, 68.560, 110.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.132 - 1.900
R-factor	0.1831
Rwork	0.182
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.132	29.132	1.950
High resolution limit [Å]	1.899	8.490	1.900
Rmerge	0.057	0.026	0.604
Rmeas	0.063	0.029	0.670
Number of reflections	36257	441	2583
<I/σ(I)>	16.3	39.26	3.05
Completeness [%]	98.8	92.5	97.1
Redundancy	5.459	4.757	5.299
CC(1/2)	0.999	0.998	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	Hexylene glycol, HEPES, PEG10000