7CO9

Ternary complex of DNA polymerase Mu with 1-nt gapped DNA (T:dGMPNPP) and Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.080, 68.540, 110.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.513 - 1.599
R-factor	0.172
Rwork	0.171
R-free	0.19620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.513	27.513	1.640
High resolution limit [Å]	1.599	7.150	1.600
Rmerge	0.039	0.026	0.264
Rmeas	0.044	0.029	0.312
Number of reflections	60405	734	4144
<I/σ(I)>	22.08	41.16	3.97
Completeness [%]	98.7	93.5	93
Redundancy	5.171	4.714	3.354
CC(1/2)	0.999	0.999	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	TRIS-HCL, PEG 3350