7CN6
T4 phage spackle protein gp61.3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.376, 46.921, 75.277 |
| Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.600 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.107 | 0.190 |
| Rmeas | 0.151 | 0.269 |
| Rpim | 0.107 | 0.190 |
| Number of reflections | 51366 | 1001 |
| <I/σ(I)> | 4 | 2.1 |
| Completeness [%] | 95.2 | 75.4 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.979 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 293.15 | PEG4000, glycine, calcium chloride |
