7CMQ

Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 88% RH.

Replaces:  5Z2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	71.063, 133.443, 144.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 1.650
R-factor	0.1701
Rwork	0.169
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wlv
RMSD bond length	0.009
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	1.750
High resolution limit [Å]	1.650	1.650
Rmerge	0.054	0.673
Rmeas	0.063	0.788
Number of reflections	82336	25541
<I/σ(I)>	13.96	2.12
Completeness [%]	99.4	98.8
Redundancy	3.8	3.7
CC(1/2)	0.998	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxthantine