7CMP

parE in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	74.016, 126.747, 96.044
Unit cell angles	90.00, 109.61, 90.00

Refinement procedure

Resolution	46.104 - 2.886
R-factor	0.2046
Rwork	0.202
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lps
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.940
High resolution limit [Å]	2.886	2.890
Number of reflections	37042	1849
<I/σ(I)>	21.2
Completeness [%]	98.5
Redundancy	3.9
CC(1/2)	0.980	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 45% v/v 2-metyl-2,4-pentanediol