7CMN

Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 88% RH.

Replaces:  5Z29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	70.889, 132.912, 144.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.230 - 1.420
R-factor	0.2011
Rwork	0.200
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wlv
RMSD bond length	0.009
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.230	1.500
High resolution limit [Å]	1.420	1.420
Rmerge	0.061	0.427
Rmeas	0.072	0.502
Number of reflections	127636	39778
<I/σ(I)>	11.67	2.7
Completeness [%]	99.4	98.7
Redundancy	3.6	3.6
CC(1/2)	0.997	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxthantine