7CMJ
Crystal structure of L.donovani Hypoxanthine-guanine phosphoribosyl transferase (HGPRT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9784 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 80.800, 80.800, 344.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.370 - 2.760 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.933 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.373 | 2.860 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.042 | 0.292 |
| Number of reflections | 17049 | 1731 |
| <I/σ(I)> | 10.15 | |
| Completeness [%] | 93.7 | 98.1 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M Sodium cacodylate, 0.2 M Ammonium sulfate, 20% PEG 4000 |
