7CMG

The Structure of the periplasmic domain of PorM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 2 21
Unit cell lengths	87.140, 98.280, 209.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.470 - 3.700
R-factor	0.2177
Rwork	0.213
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ey5
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.990	4.050
High resolution limit [Å]	3.700	3.700
Rpim	0.103	0.180
Number of reflections	19679	4681
<I/σ(I)>	6.4	4.2
Completeness [%]	99.0
Redundancy	3.8
CC(1/2)	0.974	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	14.6% (w/v) ethanol, 0.1 M Tris-HCl pH8.5, 150 mM NaCl, and 1mM taurine