7CMA

Structure of A151R from African swine fever virus Georgia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-27
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	P 32
Unit cell lengths	49.869, 49.869, 128.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.490 - 2.010
R-factor	0.2497
Rwork	0.249
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.080
High resolution limit [Å]	2.010	4.321	2.010
Rmerge	0.042	0.032	0.415
Rmeas	0.048	0.037	0.482
Rpim	0.023	0.017	0.243
Total number of observations	97465
Number of reflections	23627	2289	2345
<I/σ(I)>	23.4
Completeness [%]	99.0	94.9	98.6
Redundancy	4.1	4.3	3.7
CC(1/2)		0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	polyethylene glycol 20000, 0.1M MES pH 6.5